Substrate specificities and 13-cis-retinoic acid inhibition of human, mouse and bovine cis-retinol dehydrogenases

被引：30

作者：

Gamble, MV

Mata, NL

Tsin, AT

Mertz, JR

Blaner, WS

机构：

[1] Columbia Univ Coll Phys & Surg, Dept Med, New York, NY 10032 USA

[2] Columbia Univ, Inst Human Nutr, New York, NY 10032 USA

[3] Univ Texas, Dept Biol, San Antonio, TX 78285 USA

[4] New england Coll Optometry, Dept Biol Sci & Dis, Boston, MA 02115 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 2000年 / 1476卷 / 01期

基金：

美国国家卫生研究院;

关键词：

retinoic acid formation; retinoid; short chain dehydrogenase/reductase; vision; oxido-reductases;

D O I：

10.1016/S0167-4838(99)00232-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies of the human, mouse and bovine genes for 11-cis-retinol dehydrogenase (11cRDH) and human and mouse 9-cis-retinol dehydrogenase (9cRDH) suggest that they are homologs of the same enzyme. This conclusion is inconsistent with earlier literature indicating that 11cRDH is expressed solely in the eye and does not utilize 9-cis-retinol as a substrate. We have compared directly the kinetic properties of recombinant human and mouse 9cRDH with those of bovine 11cRDH for 9-cis- and 11-cis-retinol and investigated the inhibitory properties of 13-cis-retinoic acid on each of these enzymes. Human and mouse 9cRDH and bovine 11cRDH have very similar kinetic properties towards 9-cis- and 11-cis-retinol oxidation and they respond identically to 13-cis-retinoic acid inhibition. Our biochemical data are consistent with the conclusion that 9cRDH and 11cRDH are the same enzyme. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：3 / 8

页数：6

共 18 条

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