Endocytosis of anti-CCK-B/gastrin receptor antibody and effect on hepatoma cell lines

被引：6

作者：

Stubbs, M

Khan, K

Watson, SA

Savage, K

Dhillon, AP

Caplin, ME

机构：

[1] UCL Royal Free & Univ Coll Med Sch, Dept Histopathol, London, England

[2] UCL Royal Free & Univ Coll Med Sch, Dept Med, London, England

[3] Univ Nottingham, Queens Med Ctr, Canc Studies Unit, Nottingham NG7 2RD, England

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2002年 / 50卷 / 09期

关键词：

gastrin; CCK-B receptor; endocytosis; apoptosis; hepatocyte; tumor;

D O I：

10.1177/002215540205000908

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunotherapy has considerable potential in the treatment of cancer. Here we report on the uptake of an antibody raised against the CCK-B/Gastrin receptor (CCK-BR) by liver embryonic and liver tumor cell lines. In all five cell lines studied, expression of CCK-BR and uptake of labeled anti-CCK-BR antibody was observed. The labeled anti-CCK-BR antibody was localized in both the cytoplasm and nucleus of cells. In addition, we found a coincidence between the uptake of the labeled antibody by cells and the occurrence of apoptosis (cell death). The results suggest that antibodies directed against CCK-BR have potential for targeting and possibly destroying tumor cells bearing the receptor.

引用

页码：1213 / 1217

页数：5

共 18 条

[11]

Reubi JC, 1997, CANCER RES, V57, P1377

[12]

SAVAGE K, 1999, GASTROENTEROLOGY, V116, pS2801

[13] Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial [J].

Takayama, T ;

Sekine, T ;

Makuuchi, M ;

Yamasaki, S ;

Kosuge, T ;

Yamamoto, J ;

Shimada, K ;

Sakamoto, M ;

Hirohashi, S ;

Ohashi, Y ;

Kakizoe, T .

LANCET, 2000, 356 (9232) :802-807

[14] Tumor necrosis factor receptor and Fas signaling mechanisms [J].

Wallach, D ;

Varfolomeev, EE ;

Malinin, NL ;

Goltsev, YV ;

Kovalenko, AV ;

Boldin, MP .

ANNUAL REVIEW OF IMMUNOLOGY, 1999, 17 :331-367

[15]

Watson SA, 1998, INT J CANCER, V77, P572, DOI 10.1002/(SICI)1097-0215(19980812)77:4<572::AID-IJC17>3.3.CO

[16]

2-F

[17]

Watson SA, 2000, CANCER RES, V60, P5902

[18] GROWTH OF MOUSE HEPATOCYTES IS STIMULATED BY GASTRIN [J].

YAO, CZ ;

BOLD, RJ ;

ISHIZUKA, J ;

TOWNSEND, CM ;

THOMPSON, JC .

JOURNAL OF CELLULAR PHYSIOLOGY, 1995, 163 (03) :532-537

← 1 2 →