Rapid Determination of Quinolone Resistance in Acinetobacter spp.

被引：52

作者：

Hujer, Kristine M. ^{[2
,3
]}

Hujer, Andrea M. ^{[3
]}

Endimiani, Andrea ^{[2
,3
]}

Thomson, Jodi M. ^{[4
]}

Adams, Mark D. ^{[5
]}

Goglin, Karrie ^{[5
]}

Rather, Philip N. ^{[6
,7
]}

Pennella, Thuy-Trang D. ^{[1
]}

Massire, Christian ^{[1
]}

Eshoo, Mark W. ^{[1
]}

Sampath, Rangarajan ^{[1
]}

Blyn, Lawrence B. ^{[1
]}

Ecker, David J. ^{[1
]}

Bonomo, Robert A. ^{[2
,3
,4
]}

机构：

[1] Ibis Biosci, Carlsbad, CA 92008 USA

[2] Case Western Reserve Univ, Sch Med, Dept Med, Cleveland, OH 44106 USA

[3] Louis Stokes Cleveland Dept Vet Affairs Med Ctr, Res Serv, Cleveland, OH USA

[4] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA

[5] Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA

[6] Emory Univ, Sch Med, Dept Microbiol, Atlanta, GA USA

[7] Emory Univ, Sch Med, Dept Immunol, Atlanta, GA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 05期

基金：

美国国家卫生研究院;

关键词：

IONIZATION MASS-SPECTROMETRY; ANTIBIOTIC-RESISTANCE; UNIVERSAL BIOSENSOR; MEDICAL-CENTER; UNITED-STATES; WAR TRAUMA; BAUMANNII; MUTATIONS; GYRA; INFECTION;

D O I：

10.1128/JCM.02380-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized multidrug-resistant (MDR) Acinetobacter sp. isolates, we show that PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) and base composition analysis of PCR amplification products can quickly and accurately identify quinolone resistance mediated by mutations in the quinolone resistance-determining regions of gyrA and parC, two essential housekeeping genes. Single point mutations detected by PCR/ESI-MS in parC (found in 55/75 of the isolates) and in gyrA (found in 66/75 of the isolates) correlated with susceptibility testing and sequencing. By targeting resistance determinants that are encoded by genes with highly conserved DNA sequences (e. g., gyrA and parC), we demonstrate that PCR/ESI-MS can provide critical information for resistance determinant identification and can inform therapeutic decision making in the treatment of Acinetobacter sp. infections.

引用

页码：1436 / 1442

页数：7

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