Direct surface analysis of fungal species by matrix-assisted laser desorption/ionization mass spectrometry

被引：64

作者：

Valentine, NB ^{[1
]}

Wahl, JH ^{[1
]}

Kingsley, MT ^{[1
]}

Wahl, KL ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2002年 / 16卷 / 14期

关键词：

D O I：

10.1002/rcm.721

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study various methods of sample preparation and matrices were investigated to determine optimum collection and analysis criteria for fungal analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Intact spores and/or hyphae of Aspergillus niger, Rhizopus oryzae, Trichoderma reesei and Phanerochaete chrysosporium were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The fungal samples were applied to the MALDI sample target as untreated, sonicated, or acid/heat treated samples, or blotted directly from the fungal culture with double-stick tape. Ferulic acid or sinapinic acid matrix solution was layered over the dried samples and analyzed by MALDI-MS. Statistical analysis showed that simply using double-stick tape to collect and transfer to a MALDI sample plate typically worked as well as the other preparation methods, and required the least sample handling. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：1352 / 1357

页数：6