Apolipophorin-III in Galleria mellonella potentiates hemolymph lytic activity

被引：70

作者：

Halwani, AE ^{[1
]}

Dunphy, GB ^{[1
]}

机构：

[1] McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ H9X 3V9, Canada

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1999年 / 23卷 / 7-8期

关键词：

apolipophorin III; Enterococcus faecalis; Galleria mellonella; hemolymph; lysozyme; Micrococcus lysodeikticus;

D O I：

10.1016/S0145-305X(99)00037-3

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Heat-inactivated serum of non-immune Galleria mellonella larvae enhanced the lyric activity of larval cell-free hemolymph against Micrococcus lysodeikticus. The increase in bacterial lysis was due to a 17.2 kDa protein known previously to bind to bacterial lipopolysaccharides. The protein enhanced the lytic activity of insect cell-free hemolymph and hen lysozyme in vitro and insect hemolymph in vivo. The hydrophobic protein, which adhered to M. lysodeikticus. was identified by its amino acid sequence homology as apolipophorin-III. The titer of apolipophorin-III in 200-250 mg last instar larvae was 8.7 mg/ml of hemolymph. Apolipophorin-III did not bind to lysozyme. A possible mode of action of apolipophorin-III with lysozyme in the insect is proposed. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：563 / 570

页数：8

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