NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity

被引：29

作者：

Forgue, Paxton

Halouska, Steven

Werth, Mark

Xu, Kaimei

Harris, Steve

Powers, Robert

机构：

[1] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA

[2] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68588 USA

[3] Nebraska Wesleyan Univ, Dept Chem, Lincoln, NE 68504 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 08期

关键词：

drug discovery; NMR; metabolomics; principle component analysis; Aspergillus nidulans; purine degradation pathway; pyrimidine biosynthetic pathway;

D O I：

10.1021/pr060114v

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a general protocol for using comparative NMR metabolomics data to infer in vivo efficacy, specificity and toxicity of chemical leads within a drug discovery program. The methodology is demonstrated using Aspergillus nidulans to monitor the activity of urate oxidase and orotidine-5'-phosphate decarboxylase and the impact of 8-azaxanthine, an inhibitor of urate oxidase. 8-azaxanthine is shown to inhibit A. nidulans hyphal growth by in vivo inactivation of urate oxidase.

引用

页码：1916 / 1923

页数：8

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