A tool to visualize and evaluate data obtained by liquid chromatography-electrospray ionization-mass spectrometry

被引：75

作者：

Li, XJ ^{[1
]}

Pedrioli, PGA ^{[1
]}

Eng, J ^{[1
]}

Martin, D ^{[1
]}

Yi, EC ^{[1
]}

Lee, H ^{[1
]}

Aebersold, R ^{[1
]}

机构：

[1] Inst Syst Biol, Seattle, WA 98103 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 13期

关键词：

D O I：

10.1021/ac035375s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a software tool for visualizing data obtained from analyzing complex peptide mixtures by liquid chromatography (LC) electrospray ionization (EST) mass spectrometry (MS). The data are represented as a two-dimensional density plot. For experiments employing collision-induced dissociation (CID), links are embedded in the image to the CID spectra and the corresponding peptide sequences that are represented by the respective feature. The image provides an intuitive method to evaluate sample quality and the performance of an LC-ESI-MS system and can be used to optimize experimental conditions. Local patterns of the image can also be used to identify chemical contaminants and specific peptide features. Therefore, this software tool may have broad application in MS-based proteomics.

引用

页码：3856 / 3860

页数：5

共 22 条

[11] Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search [J].