Cloning and Characterization of Flagellin Genes and Identification of Flagellin Glycosylation from Thermophilic Bacillus Species

被引：12

作者：

Hayakawa, Jumpei ^{[1
]}

Kondoh, Yoshihide ^{[1
]}

Ishizuka, Morio ^{[1
]}

机构：

[1] Chuo Univ, Fac Sci & Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1128551, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 06期

关键词：

flagellin; glycosylation; gram-positive thermophile; Geobacillus species; LISTERIA-MONOCYTOGENES; MOTILITY; FILAMENT; REGIONS;

D O I：

10.1271/bbb.90092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Flagellin glycosylation was identified in Bacillus sp. PS3 and Geobacillus stearothermophilus. fit vivo complementation showed that these flagellin genes did not restore the motility of a Bacillus subtilis flagellin mutant, whereas the genes encoding non-glycosylated flagellin from Geobacillus kaustophilus and Bacillus sp. Kps3 restored motility. Moreover, four types of flagellins expressed in B. subtilis were not glycosylated. We speculate that glycosylation is required for flagellar filament assembly of these bacilli.

引用

页码：1450 / 1452

页数：3

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