Eluotropic strength in non-aqueous liquid chromatography with porous graphitic carbon

被引:30
作者
Gaudin, K [1 ]
Chaminade, P [1 ]
Baillet, A [1 ]
机构
[1] Univ Paris 11, Fac Pharm, Chim Analyt Lab, Grp Chim Analyt Paris Sud, F-92290 Chatenay Malabry, France
关键词
eluotropic strength; methylene selectivity; stationary phases; LC; porous graphitic carbon;
D O I
10.1016/S0021-9673(02)01123-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Porous graphitic carbon is an attractive packing for the chromatographic analysis of highly hydrocarbonaceous compounds with non-aqueous mobile phase. An eluotropic-strength scale of 10 pure organic solvents was established using the methylene selectivity from the fatty acid methyl ester homologous series (chain length between 18 and 31 carbon atoms). Eight binary mobile phases combining a weak solvent: methanol or acetonitrile with a strong solvent: toluene, chloroform, dichloromethane or tetrahydrofuran at different volume fractions phi of strong solvents (ranging from 0.3 to 1.0) were tested and their eluotropic strengths were then compared with those of pure solvents. The curves of the eluotropic strength versus the volume fraction of the strong solvent followed two different trends: linear or curved. The knowledge of the pure solvent strength is not sufficient to predict the eluotropic strength of solvent in the mixture. Then modelling of the eluotropic strength for binary mobile phases was envisaged in order to provide a prediction tool. This model was assessed for the establishment of the composition of eight iso-eluotropic mobile phases. Good assessment was found except in the case of toluene with acetonitrile where the difference between the predicted and the real value was the highest. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:61 / 68
页数:8
相关论文
共 24 条
  • [1] Analysis of cereal digalactosyldiacylglycerol molecular species by high-performance liquid chromatography
    Bergqvist, MHJ
    Kaufmann, P
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1996, 73 (02) : 211 - 217
  • [2] Liquid chromatography of polyglycerol fatty esters and fatty ethers on porous graphitic carbon and octadecyl silica by using evaporative light scattering detection and mass spectrometry
    Cassel, S
    Chaimbault, P
    Debaig, C
    Benvegnu, T
    Claude, S
    Plusquellec, D
    Rollin, P
    Lafosse, M
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 919 (01) : 95 - 106
  • [3] SELECTIVITY FOR HOMOLOGOUS SERIES IN REVERSED PHASE LIQUID-CHROMATOGRAPHY .1. THEORY
    COLIN, H
    GUIOCHON, G
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1980, 18 (02) : 54 - 63
  • [4] COMPARISON OF SOME PACKINGS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-SOLID CHROMATOGRAPHY .2. SOME THEORETICAL CONSIDERATIONS
    COLIN, H
    GUIOCHON, G
    [J]. JOURNAL OF CHROMATOGRAPHY, 1978, 158 (OCT): : 183 - 205
  • [5] THE SOLVENT ELUOTROPIC STRENGTH ON CARBON ADSORBENTS
    COLIN, H
    GUIOCHON, G
    JANDERA, P
    [J]. CHROMATOGRAPHIA, 1982, 15 (02) : 133 - 139
  • [6] REVERSED-PHASE LIQUID-SOLID CHROMATOGRAPHY ON MODIFIED CARBON-BLACK
    COLIN, H
    EON, C
    GUIOCHON, G
    [J]. JOURNAL OF CHROMATOGRAPHY, 1976, 122 (JUL7): : 223 - 242
  • [7] Mechanism of response enhancement in evaporative light scattering detection with the addition of triethylamine and formic acid
    Deschamps, FS
    Baillet, A
    Chaminade, P
    [J]. ANALYST, 2002, 127 (01) : 35 - 41
  • [8] Response enhancement for the evaporative light scattering detection for the analysis of lipid classes and molecular species
    Deschamps, FS
    Gaudin, K
    Lesellier, E
    Tchapla, A
    Ferrier, D
    Baillet, A
    Chaminade, P
    [J]. CHROMATOGRAPHIA, 2001, 54 (9-10) : 607 - 611
  • [9] DESCHAMPS FS, UNPUB
  • [10] Porous graphitized carbon and octadecyl-silica columns in the separation of some alkylglycoside detergents
    Elfakir, C
    Lafosse, M
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 782 (02) : 191 - 198