Measurement of tafenoquine (WR 238605) in human plasma and venous and capillary blood by high-pressure liquid chromatography

被引:19
作者
Kocisko, DA
Walsh, DS
Eamsila, C
Edstein, MD
机构
[1] Australian Army Malaria Inst, Dept Pharmacol, Enoggera, Qld 4052, Australia
[2] USA Med Component, Armed Forces Res Inst Med Sci, Dept Immunol & Med, Bangkok, Thailand
[3] Royal Thai Army Med Component, Armed Forces Res Inst Med Sci, Malaria Res Div, Bangkok, Thailand
关键词
tafenoquine; WR; 238605; malaria; high-pressure liquid chromatography;
D O I
10.1097/00007691-200004000-00008
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A simple, rapid, and accurate high-pressure liquid chromatographic method with fluorescence detection is described for the measurement of tafenoquine (TQ) (also known as WR 238605) from human plasma and venous and capillary blood. Tafenoquine was measured in plasma and venous blood following protein precipitation. Chromatographic separation was achieved using a Waters S5P Spherisorb phenyl analytical cartridge (150 mm x 4.6 mm ID., 5 mu m particle size) (Waters, Milford, MA, USA) and a mobile phase of 22 mM ammonium acetate, pH 4:acetonitrile (45:55, vol/vol). The flow rate was 1.5 mL/min and the retention times were similar to 3.5 min far WR VIIIAc (internal standard) and similar to 7.8 min for TQ. The interday and intraday coefficients of variation of TQ over a concentration range of 20-1000 ng/mL in plasma were less than or equal to 8.4% and in venous blood were less than or equal to 9.6%. The mean percent difference between added concentration and obtained concentration was 7.3% in plasma and 8.5% in venous blood over the corresponding concentration range. The limit of quantitation for both fluids was 10 ng/mL, Tafenoquine concentrations were comparable between capillary and venous blood with no significant difference between measurement in both biological fluids. The clinical application of the method was demonstrated by measuring plasma and whole blood concentrations of TQ from participants in a chemosuppression trial of the drug against malaria infections in Thailand.
引用
收藏
页码:184 / 189
页数:6
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