Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods

被引：35

作者：

Bes, M

Guérin-Faublée, V

Meugnier, H

Etienne, J

Freney, J

机构：

[1] Ctr Natl Reference Toxemies Staphylocoques, UPRES EA 1655, Bacteriol Lab, Fac Med RTH Laennec, F-69372 Lyon 08, France

[2] Ecole Natl Vet, Dept Sante Publ, Bacteriol Lab, F-69280 Marcy L Etoile, France

来源：

VETERINARY MICROBIOLOGY | 2000年 / 71卷 / 3-4期

关键词：

Staphylococcus sp; identification; internal transcribed spacer-polymerase chain reaction (ITS-PCR); cattle-bacteria;

D O I：

10.1016/S0378-1135(99)00163-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fifty-six Staphylococcus strains isolated from cases of bovine mammary infections were identified by using phenotypic and genotypic methods. Twenty-eight strains (50%) were identified at the species level according to their phenotypic characteristics, whereas the remaining 28 strains presented atypical or unreliable profiles. A combination of phenotypic and genotypic methods allowed the 56 strains studied to be classified, Internal transcribed spacer-polymerase chain reaction (ITS-PCR) based on the polymorphism of the 16S-23S rDNA spacer region appeared as a rapid and reliable method for the classification of bovine staphylococcal isolates at the species and subspecies levels. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：287 / 294

页数：8

共 27 条

[1]

Aarestrup FM, 1995, ACTA VET SCAND, V36, P475

[2] Prevalence and duration of intramammary infection in Danish heifers during the peripartum period [J].

Aarestrup, FM ;

Jensen, NE .

JOURNAL OF DAIRY SCIENCE, 1997, 80 (02) :307-312

[3] Exoprotein and slime production by coagulase-negative staphylococci isolated from goats' milk [J].

Bedidi-Madani, N ;

Greenland, T ;

Richard, Y .

VETERINARY MICROBIOLOGY, 1998, 59 (2-3) :139-145

[4]

BIRGERSSON A, 1991, ZBL BAKT S, V21, P331

[5] SPECIES IDENTIFICATION AND SOME CHARACTERISTICS OF COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM BOVINE UDDERS [J].

BIRGERSSON, A ;

JONSSON, P ;

HOLMBERG, O .

VETERINARY MICROBIOLOGY, 1992, 31 (2-3) :181-189

[6] A comparison of methods used in species identification of coagulase-negative staphylococci isolated from the milk of sheep [J].

Burriel, AR ;

Scott, M .

VETERINARY JOURNAL, 1998, 155 (02) :183-188

[7] A SIMPLE IDENTIFICATION SCHEME FOR COAGULASE-NEGATIVE STAPHYLOCOCCI FROM BOVINE MASTITIS [J].

DEVRIESE, LA ;

LAEVENS, H ;

HAESEBROUCK, F ;

HOMMEZ, J .

RESEARCH IN VETERINARY SCIENCE, 1994, 57 (02) :240-244

[8] A 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBE FOR THE DIFFERENTIATION OF STAPHYLOCOCCUS-HAEMOLYTICUS FROM S WARNERI AND S HOMINIS [J].

DRUEL, B ;

WEIZENEGGER, M ;

LUDWIG, W ;

FRENEY, J ;

SCHLEIFER, KH ;

FLEURETTE, J .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (04) :535-537

[9] Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions [J].

Forsman, P ;

TilsalaTimisjarvi, A ;

Alatossava, T .

MICROBIOLOGY-UK, 1997, 143 :3491-3500

[10] STAPHYLOCOCCUS-LUGDUNENSIS SP-NOV AND STAPHYLOCOCCUS SCHLEIFERI SP-NOV, 2 SPECIES FROM HUMAN CLINICAL SPECIMENS [J].

FRENEY, J ;

BRUN, Y ;

BES, M ;

MEUGNIER, H ;

GRIMONT, F ;

GRIMONT, PAD ;

NERVI, C ;

FLEURETTE, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1988, 38 (02) :168-172

← 1 2 3 →