Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centred rearrangements

被引：139

作者：

Zhang, ZH

Ren, JS

Harlos, K

McKinnon, CH

Clifton, IJ

Schofield, CJ

机构：

[1] Oxford Ctr Mol Sci, Oxford OX1 3QY, England

[2] Dyson Perrins Lab, Dept Chem, Oxford OX1 3QY, England

[3] Wellcome Trust Ctr Human Genet, Struct Biol Div, Oxford OX3 7BN, England

[4] Oxford Ctr Mol Sci, Oxford OX3 7BN, England

来源：

FEBS LETTERS | 2002年 / 517卷 / 1-3期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国工程与自然科学研究理事会;

关键词：

clavaminate synthase; clavulanic acid; dioxygenase; non-haem; 2-oxoglutarate; oxygenase;

D O I：

10.1016/S0014-5793(02)02520-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to exposure with NO, the active site Fe(II) is octahedrally coordinated by a water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the side-chains of an aspartyl and two histidinyl residues. NO binds to the position previously occupied by the 2OG 1-carboxylate concomitant with rearrangement of the latter to the position previously occupied by the displaced water. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：7 / 12

页数：6

共 26 条

[1] Chemistry and biosynthesis of clavulanic acid and other clavams
Baggaley, KH
Brown, AG
Schofield, CJ
[J]. NATURAL PRODUCT REPORTS, 1997, 14 (04) : 309 - 333
[2] INCORPORATION OF O-18-LABELED WATER INTO OXYGENATED PRODUCTS PRODUCED BY THE ENZYME DEACETOXY DEACETYLCEPHALOSPORIN C-SYNTHASE
BALDWIN, JE
ADLINGTON, RM
CROUCH, NP
PEREIRA, IAC
[J]. TETRAHEDRON, 1993, 49 (34) : 7499 - 7518
[3] A SUBSTRATE-ANALOG STUDY ON CLAVAMINIC ACID SYNTHASE - POSSIBLE CLUES TO THE BIOSYNTHETIC ORIGIN OF PROCLAVAMIC ACID
BALDWIN, JE
LLOYD, MD
WHASON, B
SCHOFIELD, CJ
ELSON, SW
BAGGALEY, KH
NICHOLSON, NH
[J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1993, (06) : 500 - 502
[4] Crystallography & NMR system:: A new software suite for macromolecular structure determination
Brunger, AT
Adams, PD
Clore, GM
DeLano, WL
Gros, P
Grosse-Kunstleve, RW
Jiang, JS
Kuszewski, J
Nilges, M
Pannu, NS
Read, RJ
Rice, LM
Simonson, T
Warren, GL
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 905 - 921
[5] EXPRESSION AND PURIFICATION OF 2 ISOZYMES OF CLAVAMINATE SYNTHASE AND INITIAL CHARACTERIZATION OF THE IRON-BINDING SITE - GENERAL ERROR ANALYSIS IN POLYMERASE CHAIN-REACTION AMPLIFICATION
BUSBY, RW
CHANG, MDT
BUSBY, RC
WIMP, J
TOWNSEND, CA
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (09) : 4262 - 4269
[6] Structure of proline 3-hydroxylase - Evolution of the family of 2-oxoglutarate dependent oxygenases
Clifton, IJ
Hsueh, LC
Baldwin, JE
Harlos, K
Schofield, CJ
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (24): : 6625 - 6636
[7] C-elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation
Epstein, ACR
Gleadle, JM
McNeill, LA
Hewitson, KS
O'Rourke, J
Mole, DR
Mukherji, M
Metzen, E
Wilson, MI
Dhanda, A
Tian, YM
Masson, N
Hamilton, DL
Jaakkola, P
Barstead, R
Hodgkin, J
Maxwell, PH
Pugh, CW
Schofield, CJ
Ratcliffe, PJ
[J]. CELL, 2001, 107 (01) : 43 - 54
[8] KINETIC STUDY OF THYMINE 7-HYDROXYLASE FROM NEUROSPORA-CRASSA
HOLME, E
[J]. BIOCHEMISTRY, 1975, 14 (22) : 4999 - 5003
[9] Targeting of HIF-α to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
Jaakkola, P
Mole, DR
Tian, YM
Wilson, MI
Gielbert, J
Gaskell, SJ
von Kriegsheim, A
Hebestreit, HF
Mukherji, M
Schofield, CJ
Maxwell, PH
Pugh, CW
Ratcliffe, PJ
[J]. SCIENCE, 2001, 292 (5516) : 468 - 472
[10] JONES TA, 1990, CRYSTALLOGRAPHIC MOD, P189

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