Toxoplasma gondii expressed sequence tags: Insight into tachyzoite gene expression

被引：43

作者：

Wan, KL ^{[1
]}

Blackwell, JM ^{[1
]}

Ajioka, JW ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT PATHOL,CAMBRIDGE CB2 1QP,ENGLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1996年 / 75卷 / 02期

基金：

英国惠康基金;

关键词：

Toxoplasma gondii; tachyzoite; expressed sequence tag; cDNA;

D O I：

10.1016/0166-6851(95)02524-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of DNA sequences from the 5' end of 239 directionally cloned Toxoplasma gondii RH strain tachyzoite derived cDNAs revealed significant similarity to several classes of genes/proteins including 24 ribosomal proteins, five metabolic enzymes, four cell-cycle regulators and 15 previously cloned T.gondii genes. The remaining sequences with no significant match include several which were recovered more than once. The variety and redundancy of expressed sequence tags (ESTs GenBankTM accession numbers T62239-T62475) in this sample suggest that the tachyzoite cDNA library reflects tachyzoite gene expression. A large scale EST effort should uncover many new genes and provide a wealth of information about genes involved with the growth and proliferation of tachyzoites.

引用

页码：179 / 186

页数：8

共 28 条

[11]

HANICJOYCE PJ, 1987, J BIOL CHEM, V262, P2845

[12] A HUMAN HOMOLOG OF THE YEAST GST1-GENE CODES FOR A GTP-BINDING PROTEIN AND IS EXPRESSED IN A PROLIFERATION-DEPENDENT MANNER IN MAMMALIAN-CELLS [J].