A novel approach for characterizing expression levels of genes duplicated by polyploidy

被引：60

作者：

Udall, Joshua A. ^{[1
]}

Swanson, Jordan M.

Nettleton, Dan

Percifield, Ryan J.

Wendel, Jonathan F.

机构：

[1] Iowa State Univ Sci & Technol, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA

[2] Iowa State Univ Sci & Technol, Dept Stat, Ames, IA 50011 USA

来源：

GENETICS | 2006年 / 173卷 / 03期

关键词：

D O I：

10.1534/genetics.106.058271

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Studying gene expression in polyploids is complicated by genomewide gene duplication and the problem of distinguishing transcript pools derived from each of the two homeologous genomes such as the A- and D-genomes of allotetraploid Gossypium. Short oligonucleotide probes designed to specifically target several hundred homeologous gene pairs of Gossypium were printed on custom NimbleGen microarrays. These results demonstrate that relative expression levels of homeologous genes may be measured by microarrays and that deviation from equal expression levels of homeologous loci may be common in the allotetraploid nucleus of Gossypium.

引用

页码：1823 / 1827

页数：5

共 18 条

[11] Understanding mechanisms of novel gene expression in polyploids [J].

Osborn, TC ;

Pires, JC ;

Birchler, JA ;

Auger, DL ;

Chen, ZJ ;

Lee, HS ;

Comai, L ;

Madlung, A ;

Doerge, RW ;

Colot, V ;

Martienssen, RA .

TRENDS IN GENETICS, 2003, 19 (03) :141-147

[12]

R Development Core Team, 2005, R LANG ENV STAT COMP

[13] Rate variation among nuclear genes and the age of polyploidy in Gossypium [J].

Senchina, DS ;

Alvarez, I ;

Cronn, RC ;

Liu, B ;

Rong, JK ;

Noyes, RD ;

Paterson, AH ;

Wing, RA ;

Wilkins, TA ;

Wendel, JF .

MOLECULAR BIOLOGY AND EVOLUTION, 2003, 20 (04) :633-643

[14] The bioperl toolkit:: Perl modules for the life sciences [J].

Stajich, JE ;

Block, D ;

Boulez, K ;

Brenner, SE ;

Chervitz, SA ;

Dagdigian, C ;

Fuellen, G ;

Gilbert, JGR ;

Korf, I ;

Lapp, H ;

Lehväslaiho, H ;

Matsalla, C ;

Mungall, CJ ;

Osborne, BI ;

Pocock, MR ;

Schattner, P ;

Senger, M ;

Stein, LD ;

Stupka, E ;

Wilkinson, MD ;

Birney, E .

GENOME RESEARCH, 2002, 12 (10) :1611-1618

[15] A global assembly of cotton ESTs [J].

Udall, JA ;

Swanson, JM ;

Haller, K ;

Rapp, RA ;

Sparks, ME ;

Hatfield, J ;

Yu, YS ;

Wu, YR ;

Dowd, C ;

Arpat, AB ;

Sickler, BA ;

Wilkins, TA ;

Guo, JY ;

Chen, XY ;

Scheffler, J ;

Taliercio, E ;

Turley, R ;

McFadden, H ;

Payton, P ;

Klueva, N ;

Allen, R ;

Zhang, DS ;

Haigler, C ;

Wilkerson, C ;

Suo, JF ;

Schulze, SR ;

Pierce, ML ;

Essenberg, M ;

Kim, H ;

Llewellyn, DJ ;

Dennis, ES ;

Kudrna, D ;

Wing, R ;

Paterson, AH ;

Soderlund, C ;

Wendel, JF .

GENOME RESEARCH, 2006, 16 (03) :441-450

[16] Genomewide nonadditive gene regulation in Arabidopsis allotetraploids [J].

Wang, JL ;

Tian, L ;

Lee, HS ;

Wei, NE ;

Jiang, HM ;

Watson, B ;

Madlung, A ;

Osborn, TC ;

Doerge, RW ;

Comai, L ;

Chen, ZJ .

GENETICS, 2006, 172 (01) :507-517

[17] Polyploidy and the evolutionary history of cotton [J].

Wendel, JF ;

Cronn, RC .

ADVANCES IN AGRONOMY, VOL 78, 2003, 78 :139-186

[18]

Wilkins Thea A., 1996, P21

← 1 2 →