Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing

被引：66

作者：

Hurley, DJ ^{[1
]}

Seaman, SE ^{[1
]}

Mazura, JC ^{[1
]}

Tor, Y ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

ORGANIC LETTERS | 2002年 / 4卷 / 14期

关键词：

D O I：

10.1021/ol026043x

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides.

引用

页码：2305 / 2308

页数：4

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