Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis

被引：61

作者：

Yang Ming-Ming

Zhang Wei-Wei

Zhang Xi-Feng

Cen Pei-Lin ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Mat Sci & Chem Engn, Inst Biochem Engn, Hangzhou 310027, Peoples R China

[2] NW A&F Univ, Coll Anim Sci, Yangling 712100, Peoples R China

[3] Yangguang Guangji Med R&D Co Lt, Wuhan 430062, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 21期

关键词：

Bacillus subtilis; expression vector; glv promoter; thermostable beta-galactosidase;

D O I：

10.1007/s10529-006-9146-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.

引用

页码：1713 / 1718

页数：6

共 21 条

[1] Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I [J].