Lateral lipid diffusion in phospholipid monolayers coupled to polyelectrolyte films

被引:14
作者
Auch, M [1 ]
Fischer, B [1 ]
Möhwald, H [1 ]
机构
[1] Max Planck Inst Kolloid & Grenzflachenforsch, D-12489 Berlin, Germany
关键词
FRAP; lateral diffusion; phospholipids; polyelectrolytes;
D O I
10.1016/S0927-7757(99)00390-8
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The lateral diffusion of lipid probes in lipid monolayers on polyelectrolyte support in different solvent environment was studied by a fringe pattern photobleaching experiment that enabled to measure diffusion coefficients D of over six orders of magnitude, In nonpolar solvents, as well as in air, electrostatic binding of ionic and also of zwitterionic lipids is so strong, that D < 10(-12) cm(2) s(-1) was observed. In polar solvents (e.g. methanol), the interaction is reduced for zwitterionic lipids, enabling D > 10(-7) cm(2) s(-1). In water, the coupling is similarly reduced for zwitterionic, not for ionic lipids. In the case of a weak interaction with the polymer, one encounters a temperature dependence for the monolayer of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as expected for a system with a fluid/gel phase transition. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:39 / 45
页数:7
相关论文
共 10 条
[1]  
BECK K, 1987, CYTOMECHANICS, pCH2
[2]  
DECHER G, 1992, PROG COLL POL SCI S, V89, P160
[3]  
Donath E, 1998, ANGEW CHEM INT EDIT, V37, P2202, DOI 10.1002/(SICI)1521-3773(19980904)37:16<2201::AID-ANIE2201>3.0.CO
[4]  
2-E
[5]  
HOUSLAY MD, 1994, DYNAMICS BIOL MEMBRA
[6]   STUDY OF POLYMER DIFFUSION ACROSS THE INTERFACE IN LATEX FILMS THROUGH DIRECT ENERGY-TRANSFER EXPERIMENTS [J].
LIU, YS ;
FENG, JR ;
WINNIK, MA .
JOURNAL OF CHEMICAL PHYSICS, 1994, 101 (10) :9096-9103
[7]  
MIEHLICH R, 1993, MUSTERBLEICHEN EVANE
[8]   FLUORESCENCE MICROPHOTOLYSIS - DIFFUSION MEASUREMENTS IN SINGLE CELLS [J].
PETERS, R .
NATURWISSENSCHAFTEN, 1983, 70 (06) :294-302
[9]   DETERMINATION OF MOLECULAR-MOTION IN MEMBRANES USING PERIODIC PATTERN PHOTOBLEACHING [J].
SMITH, BA ;
MCCONNELL, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) :2759-2763
[10]   Layer-by-layer self assembly of polyelectrolytes on colloidal particles [J].
Sukhorukov, GB ;
Donath, E ;
Lichtenfeld, H ;
Knippel, E ;
Knippel, M ;
Budde, A ;
Mohwald, H .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1998, 137 (1-3) :253-266