Suppression of a key gene involved in chlorophyll biosynthesis by means of virus-inducing gene silencing

被引:41
作者
Hiriart, JB [1 ]
Lehto, K [1 ]
Tyystjärvi, E [1 ]
Junttila, T [1 ]
Aro, EM [1 ]
机构
[1] Turku Univ, Dept Biol, Lab Plant Physiol & Mol Biol, Washington, DC 20540 USA
基金
芬兰科学院;
关键词
chlorophyll biosynthesis; magnesium chelatase; tobacco mosaic virus; virus-induced gene silencing;
D O I
10.1023/A:1016000627231
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ChlH gene coding the H subunit of magnesium chelatase, an enzyme involved in chlorophyll biosynthesis, was silenced in Nicotiana benthamiana plants by infection with tobacco mosaic virus vectors (pTMV-30b) containing 67, 214 or 549 nt long ChlH inserts. Silencing of the nuclear ChlH gene induced a chimeric phenotype with green and yellow/white leaves associated with alterations of chloroplast shape and ultrastructure. The symptoms became first evident around veins of young leaves, and only later in the mesophyll tissues. The efficiency of gene silencing was not dependent on the insert orientation, but was strongly correlated with the size of the ChlH insert, providing a flexible method to modulate the level of gene suppression. Silencing efficiency seemed to be strongly dependent on endogenous ChlH mRNA level of the target tissue. Silencing of the ChlH gene with the longest fragment of 549 nt also lowered the accumulation of ChlD and chlorophyll synthetase mRNAs, i.e. other genes involved in chlorophyll biosynthesis.
引用
收藏
页码:213 / 224
页数:12
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