Involvement of an efflux system in mediating high level of fluoroquinolone resistance in Mycobacterium smegmatis

被引：31

作者：

Banerjee, SK ^{[1
]}

Bhatt, K ^{[1
]}

Rana, S ^{[1
]}

Misra, P ^{[1
]}

Chakraborti, PK ^{[1
]}

机构：

[1] INST MICROBIOL TECHNOL,DIV MOL BIOL,CHANDIGARH 160036,INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 226卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.1362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A wild type strain of Mycobacterium smegmatis mc(2) 155 was serially adapted to 64 fold of minimal inhibitory concentration of an antimycobacterial agent, ciprofloxacin. This clone (CIPr) exhibited cross resistance to ofloxacin and ethidium bromide. The rate of drug efflux was accelerated in CIPr compared to the wild type strain. Verapamil, a calcium channel blocker, enhanced the drug accumulation in CIPr by diminishing the efflux and thus reversed the resistant phenotype. Additionally, a missense mutation was detected in the quinolone resistance determining region of the DNA-gyrase A subunit of CIPr. Taken together, these results suggest that drug efflux plays a major role in conferring such a high level of resistance in CIPr, in addition to the mutation in the DNA-gyrase locus. (C) 1996 Academic Press, Inc.

引用

页码：362 / 368

页数：7

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