Smart PEGylated gold nanoparticles for the cytoplasmic delivery of siRNA to induce enhanced gene silencing

被引：118

作者：

Oishi, Motoi

Nakaogami, Junpei

Ishii, Takehiko

Nagasaki, Yukio

机构：

[1] Univ Tsukuba, Tsukuba Res Ctr Interdisciplinary Mat Sci, Tsukuba, Ibaraki 3058573, Japan

[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan

来源：

CHEMISTRY LETTERS | 2006年 / 35卷 / 09期

关键词：

D O I：

10.1246/cl.2006.1046

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel cytoplasmic delivery system of siRNA was developed by complexation between poly(ethylene glycol)-blockpoly(2-(N,N-dimethylamino)ethyl methacrylate) copolymer (PEG-PAMA) and gold nanoparticles (GNPs), followed by the immobilization of siRNA bearing a thiol group (SH-siRNA). The PEGylated GNPs containing SH-siRNA showed a significant RNAi activity in HuH-7 cells.

引用

页码：1046 / 1047

页数：2

共 15 条

[1] Selective synthesis of heterobifunctional poly(ethylene glycol) derivatives containing both mercapto and acetal terminals
Akiyama, Y
Otsuka, H
Nagasaki, Y
Kato, M
Kataoka, K
[J]. BIOCONJUGATE CHEMISTRY, 2000, 11 (06) : 947 - 950
[2] Biodistribution of phosphodiester and phosphorothioate siRNA
Braasch, DA
Paroo, Z
Constantinescu, A
Ren, G
Öz, OK
Mason, RP
Corey, DR
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2004, 14 (05) : 1139 - 1143
[3] Single-mismatch detection using gold-quenched fluorescent oligonucleotides
Dubertret, B
Calame, M
Libchaber, AJ
[J]. NATURE BIOTECHNOLOGY, 2001, 19 (04) : 365 - 370
[4] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, SM
Harborth, J
Lendeckel, W
Yalcin, A
Weber, K
Tuschl, T
[J]. NATURE, 2001, 411 (6836) : 494 - 498
[5] GLYCOSYLATED POLYLYSINE DNA COMPLEXES - GENE-TRANSFER EFFICIENCY IN RELATION WITH THE SIZE AND THE SUGAR SUBSTITUTION LEVEL OF GLYCOSYLATED POLYLYSINES AND WITH THE PLASMID SIZE
ERBACHER, P
ROCHE, AC
MONSIGNY, M
MIDOUX, P
[J]. BIOCONJUGATE CHEMISTRY, 1995, 6 (04) : 401 - 410
[6] Glutathione-mediated delivery and release using monolayer protected nanoparticle carriers
Hong, R
Han, G
Fernández, JM
Kim, BJ
Forbes, NS
Rotello, VM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (04) : 1078 - 1079
[7] Preparation of functionally PEGylated gold nanoparticles with narrow distribution through autoreduction of auric cation by α-biotinyl-PEG-block-[poly(2-(N,N-dimethylamino)ethyl methacrylate)]
Ishii, T
Otsuka, H
Kataoka, K
Nagasaki, Y
[J]. LANGMUIR, 2004, 20 (03) : 561 - 564
[8] Organic-inorganic hybrid-nanocarrier of siRNA constructing through the self-assembly of calcium phosphate and PEG-based block aniomer
Kakizawa, Y
Furukawa, S
Ishii, A
Kataoka, K
[J]. JOURNAL OF CONTROLLED RELEASE, 2006, 111 (03) : 368 - 370
[9] Block copolymer-coated calcium phosphate nanoparticles sensing intracellular environment for oligodeoxynucleotide and siRNA delivery
Kakizawa, Y
Furukawa, S
Kataoka, K
[J]. JOURNAL OF CONTROLLED RELEASE, 2004, 97 (02) : 345 - 356
[10] Polyion complex micelles with reactive aldehyde groups on their surface from plasmid DNA and end-functionalized charged blocks copolymers
Kataoka, K
Harada, A
Wakebayashi, D
Nagasaki, Y
[J]. MACROMOLECULES, 1999, 32 (20) : 6892 - 6894

← 1 2 →