Terminal deoxynucleotidyl transferase catalyzes the reaction of DNA phosphorylation

被引：27

作者：

Arzumanov, AA

Victorova, LS

Jasko, MV

Yesipov, DS

Krayevsky, AA

机构：

[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia

[2] Univ Oslo, Ctr Med Studies, Moscow 117984, Russia

[3] Moscow MV Lomonosov State Univ, Interfac Lab, Moscow 119899, Russia

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 05期

关键词：

D O I：

10.1093/nar/28.5.1276

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reaction of phosphorylation and phosphonylation of an oligodeoxynucleotide 3'-terminal hydroxyl (oligodeoxynucleotidyl kinase activity) catalyzed by calf thymus terminal deoxynucleotidyl transferase (TDT) was found. Triphosphates modified at P alpha-, P alpha,gamma- P alpha,beta,gamma-residues served as low-molecular weight substrates, The reaction was TDT specific; human DNA polymerases alpha and beta, as well as AMV reverse transcriptase did not catalyze it. The donor activity of modified triphosphates or triphosphonates depended on their structure and was increased with an increase in their hydrophobicity, The substrate activity of some modified triphosphates was up to one order of magnitude higher than that of ddTTP.

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页码：1276 / 1281

页数：6

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