Bioanalytical applications of aptamer and molecular-beacon probes in fluorescence-affinity assays

被引：52

作者：

Goulko, Alevtina A. ^{[1
]}

Li, Feng ^{[1
]}

Le, X. Chris ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G3, Canada

[2] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2G3, Canada

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2009年 / 28卷 / 07期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Affinity probe; Aptamer; Bioanalysis; Capillary electrophoresis; Fluorescence; Fluorescence decay life-time; Fluorescence polarization; Fluorescence-resonance-energy transfer; Molecular beacon; Nucleic acid; ROLLING CIRCLE AMPLIFICATION; RESONANCE ENERGY-TRANSFER; KINETIC CAPILLARY-ELECTROPHORESIS; PROXIMITY LIGATION ASSAYS; PROTEIN-DNA INTERACTIONS; NUCLEIC-ACID ANALYSIS; QUANTUM DOTS; CONJUGATED NANOPARTICLES; POLARIZATION DETECTION; ANTIGEN-DETECTION;

D O I：

10.1016/j.trac.2009.03.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This critical review summarizes recent developments in highly sensitive, specific assays using nucleic-acid (NA)-affinity probes and fluorescence detection. We emphasize two groups of DNA and RNA probes (i.e. aptamers and molecular beacons) because of the increase in their bioanalytical applications. The affinity and the specificity of these NA probes combined with the diverse detection capability of fluorescence measurements (e.g., intensity, polarization, resonance-energy transfer and decay life-time) enable ultrasensitive assays for proteins, gene mutations, single-nucleotide polymorphisms and molecular-binding events. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：878 / 892

页数：15

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