Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)

被引：54

作者：

Richter, A.

de Kathen, A.

de Lorenzo, G.

Briviba, K.

Hain, R.

Ramsay, G.

Jacobsen, H. -J.

Kiesecker, H.

机构：

[1] German Collect Microorganisms & Cell Cultures Gmb, D-38124 Braunschweig, Germany

[2] Leibniz Univ Hannover, Dept Mol Genet, D-30419 Hannover, Germany

[3] Kathen & Pickardt BioTechConsult GbR, D-10627 Berlin, Germany

[4] Univ Roma La Sapienza, Dipartimento Biol Vegetale, I-00185 Rome, Italy

[5] Fed Res Ctr Nutr, Inst Nutr Physiol, D-76131 Karlsruhe, Germany

[6] Bayer CropSci GmbH, Global Biol Herbicides Biochem, D-65926 Frankfurt, Germany

[7] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

来源：

PLANT CELL REPORTS | 2006年 / 25卷 / 11期

关键词：

Agrobacterium; expression stability; pea; PGIP; resveratrol;

D O I：

10.1007/s00299-006-0172-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated.

引用

页码：1166 / 1173

页数：8

共 51 条

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