Automated, high-resolution cellular retention and uptake studies in vitro

被引：70

作者：

Bjorke, Henrik ^{[1
]}

Andersson, Karl ^{[1
]}

机构：

[1] Uppsala Univ, Div Biomed Radiat Sci, Uppsala, Sweden

来源：

APPLIED RADIATION AND ISOTOPES | 2006年 / 64卷 / 08期

关键词：

cellular uptake; cellular retention; protein interactions; kinetics;

D O I：

10.1016/j.apradiso.2006.03.002

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：901 / 905

页数：5

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