A ratiometric CdSe/ZnS nanocrystal pH sensor

被引：460

作者：

Snee, Preston T. ^{[1
]}

Somers, Rebecca C. ^{[1
]}

Nair, Gautham ^{[1
]}

Zimmer, John P. ^{[1
]}

Bawendi, Moungi G. ^{[1
]}

Nocera, Daniel G. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 41期

关键词：

D O I：

10.1021/ja0618999

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The development of a reversible chemical sensor based on a CdSe/ZnS nanocrystal (NC) is described. Signal transduction is accomplished by fluorescence resonance energy transfer (FRET) between the NC and a fluorescent pH-sensitive squaraine dye attached to the surface of the NC. The efficiency of FRET, and consequently the relative intensity of NC and dye emissions, is modulated with the pH-dependent absorption cross section of the squaraine dye. The design of a NC sensor based on FRET results in a ratiometric sensor since the emission intensities of dye and NC may be referenced to the isosbestic point between NC and dye emissions. The ratiometric approach allows sensing to be performed, regardless of issues surrounding collection efficiency (scattering environment, light fluctuations, etc.) and dye:NC loadings. Copyright © 2006 American Chemical Society.

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页码：13320 / 13321

页数：2

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