In vivo gene gun-mediated DNA delivery into rodent brain tissue

被引：23

作者：

Sato, H

Hattori, S

Kawamoto, S

Kudoh, I

Hayashi, A

Yamamoto, I

Yoshinari, M

Minami, M

Kanno, H

机构：

[1] Yokohama City Univ, Sch Med, Dept Neurosurg, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Yokohama City Univ, Sch Med, Dept Anesthesiol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[3] Yokohama City Univ, Sch Med, Dept Bacteriol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[4] Yokohama City Univ, Sch Med, Dept Parasitol & Immunol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 01期

关键词：

D O I：

10.1006/bbrc.2000.2369

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various types of gene transfer into live tissues have been tried. However, in vivo gene transfer into brain tissue or neuronal cells without virus vector has required a great effort. Particle-mediated gene transfer into live brain tissue was thought to be impossible because of its fragility and the mechanical problem of a previous type of gene gun In addition, particle-mediated DNA transfer into monolayer-cultured cells without mechanical damage has been difficult. We successfully transferred DNA into rodent live brain tissue and also into monolayer-cultured cells without mechanical damage by using a new type of gene gun and also confirmed gene expression in the brain. This new method represents another variation of gene transfer into the brain, (C) 2000 Academic Press.

引用

页码：163 / 170

页数：8

共 22 条

[21] Bacterial beta-galactosidase and human dystrophin genes are expressed in mouse skeletal muscle fibers after ballistic transfection [J].