Direct identification of stylet secreted proteins from root-knot nematodes by a proteomic approach

被引:103
作者
Jaubert, S
Ledger, TN
Laffaire, JB
Piotte, C
Abad, P
Rosso, MN
机构
[1] INRA, Unite Interact Plantes Microorganismes & Sante Ve, F-06606 Antibes, France
[2] INRA, UMR ROSE, F-06606 Antibes, France
关键词
stylet secretions; root-knot nematode; calreticulin;
D O I
10.1016/S0166-6851(02)00034-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stylet secretions produced by plant parasitic root-knot nematodes are thought to be pathogenicity factors involved in the invasion of the root tissue and in the induction and maintenance of feeding cells. A new procedure was established that allowed the direct qualitative analysis of proteins secreted by Meloidogyne incognita infective juveniles. Purified proteins whose isoelectric point (pI) ranged from 5.0 to 7.5 were separated by two-dimensional (2D) electrophoresis and the seven most abundant proteins were identified by micro-sequencing. A calreticulin (CRT) was isolated and transcription of its gene in infective juveniles and adults was demonstrated. Moreover, evidence for expression of the CRT in the subventral oesophageal glands of infective juveniles was obtained. The potential roles of this secreted protein in pathogenesis and the advantages of developing this strategy to obtain new insights into plant-nematode interactions are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:205 / 211
页数:7
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