Induction of Hsp 70 in Vero cells in response to mycotoxins - Cytoprotection by sub-lethal heat shock and by Vitamin E

被引:78
作者
El Golli, Emna
Hassen, Wafa
Bouslimi, Amel
Bouaziz, Chayma
Ladjimi, M. Moncef
Bacha, Hassen
机构
[1] Fac Dent, Lab Res Biol Compatible Cpds, Monastir 5000, Tunisia
[2] Univ Versailles, CNRS, UMR 8159, Lab Genet & Cellular Biol, F-7803 Versailles, France
关键词
mycotoxins; Hsp; 70; expression; cytoprotection; vitamin E; oxidative stress;
D O I
10.1016/j.toxlet.2006.06.004
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
This paper analysed the toxicity mechanisms of several mycotoxins using Hsp 70 expression, cytoprotection of Vero cells by sub-lethal beat shock (sub-LHS) and Vitamin E. Our aim was (i) to determine whether Citrinin (CTN), Zearalenone (ZEN) and T2 toxin (T2) could induce the expression of Hsp 70, (ii) to check whether or not elevated levels of Hsp and Vitamin E pre-treatment could provide cytoprotection from these mycotoxins, and finally (iii) to emphasize the eventual involvement of oxidative stress on mycotoxin's toxicity. Our study demonstrated that the three examined mycotoxins induced Hsp 70 expression in a dose-dependent manner. A cytoprotective effect of Hsp 70 was obtained when Vero cells were exposed to sub-lethal heat shock followed by a 12 It recovery prior to mycotoxins treatment and evidenced by a reduction of their cytolethality. This cytoprotection suggested that Hsp 70 might constitute an important cellular defence mechanism. A cytoprotective action was also obtained although at lesser extent, when cells were pre-treated with an antioxidant agent, the Vitamin E before mycotoxins treatment. This Vitamin E cytoprotection evoked the involvement of oxidative stress in mycotoxins induced toxicity, which was further, confirmed by the reduction of Hsp 70 expression when cells were pre-treated with Vitamin E prior to mycotoxins. Our data clearly shows that oxidative stress is certainly involved in the toxicity of the three studied mycotoxins, Citrinin, Zearalenone and T2 toxin and may therefore constitutes a relevant part in their toxicities; however, at variable extent from one mycotoxin to another. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:122 / 130
页数:9
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