Detection and identification of aqueous saccharides by using surface-enhanced Raman spectroscopy

被引：79

作者：

Mrozek, MF ^{[1
]}

Weaver, MJ ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 16期

关键词：

D O I：

10.1021/ac020115g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The sensitive detection and characterization of carbohydrates by means of a strategy based on surface-enhanced Raman spectroscopy is demonstrated. Spectra are obtained after injecting a small amount of saccharide solution onto a roughened silver substrate, with subsequent deposition of silver colloid. The sensitivity achieved by this two-step approach enables high-quality Raman spectra to be obtained for small amounts of aqueous saccharides (5 muL of a 10(-2) M solution) utilizing minimal laser power and small signal acquisition times (a few seconds). Spectral "fingerprints" obtained for seven structurally similar monosaccharides demonstrate clearly an effective means by which each sugar can be identified. The application to more complex analyses is demonstrated for monosaccharide mixtures and a disaccharide, whereby the SERS fingerprints aid in the determination of components.

引用

页码：4069 / 4075

页数：7

共 46 条

[1] Raman spectroscopy as a discovery tool in carbohydrate chemistry [J].