The design, synthesis and characterization of a novel acceptor for real time polymerase chain reaction using both computational and experimental approaches

被引：17

作者：

Benzi, Caterina ^{[1
,2
]}

Bertolino, Chiara A. ^{[3
]}

Miletto, Ivana ^{[1
,2
]}

Ponzio, Paola ^{[3
]}

Barolo, Claudia ^{[2
,4
]}

Viscardi, Guido ^{[2
,4
]}

Coluccia, Salvatore ^{[1
,2
]}

Caputo, Giuseppe ^{[1
,2
,3
]}

机构：

[1] Univ Turin, Dipartimento Chim IFM, I-10125 Turin, Italy

[2] Univ Turin, NIS Ctr Excellence, I-10125 Turin, Italy

[3] Cyanine Technol Srl, I-10135 Turin, Italy

[4] Univ Turin, Dipartimento Chim Gen & Chim Organ, I-10125 Turin, Italy

来源：

DYES AND PIGMENTS | 2009年 / 83卷 / 01期

关键词：

Indocyanine; DFT; Time-dependent DFT; Fluorescence quenching; PCR pair; Nitro-indocyanine; DENSITY-FUNCTIONAL THEORY; EFFICIENT IMPLEMENTATION; CYANINE DYES; DNA; ABSORPTION; SPECTRA; AMPLIFICATION;

D O I：

10.1016/j.dyepig.2009.04.001

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The design of a novel, fluorescence acceptor for use in real time polymerase chain reaction analysis was carried out with the help of in-depth computational analysis of structure/property relationships. A functionalized indocyanine dye was synthesized in order to quench a fluorescent cyanine dye. The pentamethine indocyanine dye was functionalized with nitro groups so as to enhance its quenching abilities; ad hoc substituents were used to enable conjugation with DNA strands. The visible absorption and photoemission of the indocyanine dye as well as that of an unsubstituted indodicarbocyanine dye, employed as a reference model chromogenic system, were addressed both theoretically and experimentally. A Forster resonance energy transfer analysis was simulated experimentally to evaluate the quenching efficiency of the donor/acceptor couple. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：111 / 120

页数：10

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