ADAM10 Regulates Notch Function in Intestinal Stem Cells of Mice

被引:91
作者
Tsai, Yu-Hwai [1 ]
VanDussen, Kelli L. [2 ]
Sawey, Eric T. [4 ]
Wade, Alex W. [1 ]
Kasper, Chelsea [1 ]
Rakshit, Sabita [1 ]
Bhatt, Riha G. [1 ]
Stoeck, Alex [1 ]
Maillard, Ivan [3 ]
Crawford, Howard C. [5 ]
Samuelson, Linda C. [2 ]
Dempsey, Peter J. [1 ,2 ]
机构
[1] Univ Michigan, Dept Pediat & Communicable Dis, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA
[4] SUNY Stony Brook, Dept Pharmacol Sci, Stony Brook, NY 11794 USA
[5] Mayo Clin, Dept Canc Biol, Jacksonville, FL 32224 USA
基金
美国国家卫生研究院;
关键词
CBC; Intestinal Epithelium; Development; Differentiation; ATONAL HOMOLOG 1; PROGENITOR CELLS; SECRETORY PROGENITOR; IN-VIVO; CRYPT; DIFFERENTIATION; LGR5; PROLIFERATION; HOMEOSTASIS; CLEAVAGE;
D O I
10.1053/j.gastro.2014.07.003
中图分类号
R57 [消化系及腹部疾病];
学科分类号
100201 [内科学];
摘要
BACKGROUND & AIMS: A disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) is a cell surface shed-dase that regulates physiologic processes, including Notch signaling. ADAM10 is expressed in all intestinal epithelial cell types, but the requirement for ADAM10 signaling in crypt homeostasis is not well defined. METHODS: We analyzed intestinal tissues from mice with constitutive (Vil-Cre;Adam10(f/f) mice) and conditional (Vil-CreER;Adam10(f/f) and Leucine-rich repeat-containing GPCR5 [Lgr5]-CreER;Adam10(f/f) mice) deletion of ADAM10. We performed cell lineage-tracing experiments in mice that expressed a gain-of-function allele of Notch in the intestine (Rosa26(NICD)), or mice with intestine-specific disruption of Notch (Rosa26(DN-MAML)), to examine the effects of ADAM10 deletion on cell fate specification and intestinal stem cell maintenance. RESULTS: Loss of ADAM10 from developing and adult intestine caused lethality associated with altered intestinal morphology, reduced progenitor cell proliferation, and increased secretory cell differentiation. ADAM10 deletion led to the replacement of intestinal cell progenitors with 2 distinct, post-mitotic, secretory cell lineages: intermediate-like (Paneth/goblet) and enteroendocrine cells. Based on analysis of Rosa26(NICD) and Rosa26(DN-MAML) mice, we determined that ADAM10 controls these cell fate decisions by regulating Notch signaling. Cell lineage-tracing experiments showed that ADAM10 is required for survival of Lgr5(+) crypt-based columnar cells. Our findings indicate that Notch-activated stem cells have a competitive advantage for occupation of the stem cell niche. CONCLUSIONS: ADAM10 acts in a cell autonomous manner within the intestinal crypt compartment to regulate Notch signaling. This process is required for progenitor cell lineage specification and crypt-based columnar cell maintenance.
引用
收藏
页码:822 / U195
页数:26
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