Bone marrow mesenchymal stem cells ameliorate colitis-associated tumorigenesis in mice

被引：62

作者：

Chen, Zexian ^{[1
]}

He, Xiaowen ^{[1
]}

He, Xiaosheng ^{[1
]}

Chen, Xiuting ^{[1
]}

Lin, Xutao ^{[1
]}

Zou, Yifeng ^{[1
]}

Wu, Xiaojian ^{[1
]}

Lan, Ping ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Colorectal & Anal Surg, Guangzhou 510655, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 450卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Inflammatory bowel disease; Tumorigenesis; Mesenchymal stem cells; Colitis; Mice; INFLAMMATORY-BOWEL-DISEASE; COMPLEX PERIANAL FISTULA; COLORECTAL-CANCER; CROHNS-DISEASE; CLINICAL-TRIAL; STROMAL CELLS; CARCINOGENESIS; ANGIOGENESIS; SURVIVAL; THERAPY;

D O I：

10.1016/j.bbrc.2014.07.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Background and Aims: Bone marrow-derived mesenchymal stem cell (MSC) is widely studied in inflammatory bowel disease (IBD) in basic and clinical research. However, patients with IBD have higher risk of developing colorectal cancer and MSC has dual effect on tumorigenesis. This study aims to evaluate the role of MSC on tumorigenesis of IBD. Methods: MSCs were isolated from the bone marrow of allogenic mice and identified by flow cytometry. Mice in the model of colitis-associated tumorigenesis induced by azoxymethane and dextran sulfate sodium were injected with MSCs. Colon length, spleen size and tumors formation were assessed macroscopically. Pro-inflammatory cytokines and STAT3 phosphorylation in colon tissues were analyzed. Results: MSCs ameliorated the severity of colitis associated tumorigenesis compared with PBS control, with attenuated weight loss, longer colons and smaller spleens. Tumor number and tumor load were significantly less in the MSC group while tumor size remained comparable. Histological assessment indicated MSCs could reduce histological damage of the colon tissue. Decreased expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta and IL-6), and down-regulation of STAT3 phosphorylation in colon tissue were found after MSC treatment. Conclusion: MSCs might ameliorate the tumorigenesis of inflammatory bowel disease by suppression of expression of pro-inflammatory cytokines and STAT3 activation. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：1402 / 1408

页数：7

共 34 条

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