Organoid culture systems for prostate epithelial and cancer tissue

被引：533

作者：

Drost, Jarno ^{[1
,2
,3
]}

Karthaus, Wouter R. ^{[4
]}

Gao, Dong ^{[4
]}

Driehuis, Else ^{[1
,2
,3
]}

Sawyers, Charles L. ^{[4
]}

Chen, Yu ^{[4
,5
,6
,7
]}

Clevers, Hans ^{[1
,2
,3
]}

机构：

[1] Royal Netherlands Acad Arts & Sci KNAW, Hubrecht Inst, Utrecht, Netherlands

[2] Univ Med Ctr UMC Utrecht, Utrecht, Netherlands

[3] UMC Utrecht, Canc Genom Netherlands, Utrecht, Netherlands

[4] Mem Sloan Kettering Canc Ctr, Human Oncol & Pathogenesis Program, 1275 York Ave, New York, NY 10021 USA

[5] Weill Cornell Med Coll, Dept Med, New York, NY USA

[6] New York Presbyterian Hosp, New York, NY USA

[7] Mem Sloan Kettering Canc Ctr, Dept Med, 1275 York Ave, New York, NY 10021 USA

来源：

NATURE PROTOCOLS | 2016年 / 11卷 / 02期

关键词：

STEM-CELLS; MURINE PROSTATE; PROGENITOR CELLS; IN-VITRO; REGENERATION; EXPANSION; VIVO;

D O I：

10.1038/nprot.2016.006

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy mouse and human prostate cells (either bulk or FACS-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumor cells. Organoids derived from healthy material contain the differentiated luminal and basal cell types, whereas organoids derived from prostate cancer tissue mimic the histology of the tumor. We explain how to establish these cultures in the fully defined serum-free conditioned medium that is required to sustain organoid growth. Starting with the plating of digested tissue material, full-grown organoids can usually be obtained in similar to 2 weeks. The culture protocol we describe here is currently the only one that allows the growth of both the luminal and basal prostatic epithelial lineages, as well as the growth of advanced prostate cancers. Organoids established using this protocol can be used to study many different aspects of prostate biology, including homeostasis, tumorigenesis and drug discovery.

引用

页码：347 / 358

页数：12

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