The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies

被引：25

作者：

Foster, Jeremy M. ^{[1
]}

Raverdy, Sylvine ^{[1
]}

Ganatra, Mehul B. ^{[1
]}

Colussi, Paul A. ^{[1
]}

Taron, Christopher H. ^{[1
]}

Carlow, Clotilde K. S. ^{[1
]}

机构：

[1] New England Biolabs Inc, Ipswich, MA 01938 USA

来源：

PARASITOLOGY RESEARCH | 2009年 / 104卷 / 05期

关键词：

ALKALINE-PHOSPHATASE; TRYPANOSOMA-BRUCEI; MECHANISM; GENE; CATALYSIS;

D O I：

10.1007/s00436-008-1287-7

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Phosphoglycerate mutases (PGM) interconvert 2- and 3-phosphoglycerate in the glycolytic and gluconeogenic pathways. A putative cofactor-independent phosphoglycerate mutase gene (iPGM) was identified in the genome sequence of the Wolbachia endosymbiont from the filarial nematode, Brugia malayi (wBm). Since iPGM has no sequence or structural similarity to the cofactor-dependent phosphoglycerate mutase (dPGM) found in mammals, it may represent an attractive Wolbachia drug target. In the present study, wBm-iPGM cloned and expressed in Escherichia coli was mostly insoluble and inactive. However, the protein was successfully produced in the yeast Kluyveromyces lactis and the purified recombinant wBm-iPGM showed typical PGM activity. Our results provide a foundation for further development of wBm-iPGM as a promising new drug target for novel anti-filarial therapies that selectively target the endosymbiont.

引用

页码：1047 / 1052

页数：6

共 29 条

[1]

PHYLOGENY AND ONTOGENY OF THE PHOSPHOGLYCERATE MUTASES .4. DISTRIBUTION OF GLYCERATE-2,3-P2 DEPENDENT AND INDEPENDENT PHOSPHOGLYCERATE MUTASES IN ALGAE, FUNGI, PLANTS AND ANIMALS [J].

CARRERAS, J ;

MEZQUITA, J ;

BOSCH, J ;

BARTRONS, R ;

PONS, G .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1982, 71 (04) :591-597

[2]

Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus [J].

Chander, M ;

Setlow, P ;

Lamani, E ;

Jedrzejas, MJ .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 126 (02) :156-165

[3]

Trypanosoma brucei contains a 2,3-bisphosphoglycerate independent phosphoglycerate mutase [J].

Chevalier, N ;

Rigden, DJ ;

Van Roy, J ;

Opperdoes, FR ;

Michels, PAM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (05) :1464-1472

[4]

Collet JF, 2001, FEMS MICROBIOL LETT, V204, P39, DOI 10.1111/j.1574-6968.2001.tb10859.x

[5]

Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei [J].

Djikeng, Appolinaire ;

Raverdy, Sylvine ;

Foster, Jeremy ;

Bartholomeu, Daniella ;

Zhang, Yinhua ;

El-Sayed, Najib M. ;

Carlow, Clotilde .

PARASITOLOGY RESEARCH, 2007, 100 (04) :887-892

[6]

The Wolbachia genome of Brugia malayi:: Endosymbiont evolution within a human pathogenic nematode [J].

Foster, J ;

Ganatra, M ;

Kamal, I ;

Ware, J ;

Makarova, K ;

Ivanova, N ;

Bhattacharyya, A ;

Kapatral, V ;

Kumar, S ;

Posfai, J ;

Vincze, T ;

Ingram, J ;

Moran, L ;

Lapidus, A ;

Omelchenko, M ;

Kyrpides, N ;

Ghedin, E ;

Wang, S ;

Goltsman, E ;

Joukov, V ;

Ostrovskaya, O ;

Tsukerman, K ;

Mazur, M ;

Comb, D ;

Koonin, E ;

Slatko, B .

PLOS BIOLOGY, 2005, 3 (04) :599-614

[7]

Construction of bacterial artificial chromosome libraries from the parasitic nematode Brugia malayi and physical mapping of the genome of its Wolbachia endosymbiont [J].

Foster, JM ;

Kumar, S ;

Ganatra, MB ;

Kamal, IH ;

Ware, J ;

Ingram, J ;

Pope-Chappell, J ;

Guiliano, D ;

Whitton, C ;

Daub, J ;

Blaxter, ML ;

Slatko, BE .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2004, 34 (06) :733-746

[8]

FOTHERGILLGILMORE LA, 1989, ADV ENZYMOL RAMB, V62, P227

[9]

The two analogous phosphoglycerate mutases of Escherichia coli [J].

Fraser, HI ;

Kvaratskhelia, M ;

White, MF .

FEBS LETTERS, 1999, 455 (03) :344-348

[10]

A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate [J].

Gallagher, Larry A. ;

Ramage, Elizabeth ;

Jacobst, Michael A. ;

Kaul, Rajinder ;

Brittnacher, Mitchell ;

Manoil, Colin .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (03) :1009-1014

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