Porphyrin derivatives as photosensitizers for the inactivation of Bacillus cereus endospores

被引:67
作者
Oliveira, A. [1 ,2 ]
Almeida, A. [1 ,2 ]
Carvalho, C. M. B. [3 ]
Tome, J. P. C. [3 ]
Faustino, M. A. F. [3 ]
Neves, M. G. P. M. S. [3 ]
Tome, A. C. [3 ]
Cavaleiro, J. A. S. [3 ]
Cunha, A. [1 ,2 ]
机构
[1] Univ Aveiro, Dept Biol, P-3810093 Aveiro, Portugal
[2] Univ Aveiro, CESAM, P-3810093 Aveiro, Portugal
[3] Univ Aveiro, Dept Chem, P-3810093 Aveiro, Portugal
关键词
Bacillus cereus; bacterial endospores; photodynamic inactivation; porphyrins; CLOSTRIDIUM-PERFRINGENS; ANTIBACTERIAL ACTIVITY; CATIONIC PORPHYRINS; PHOTOINACTIVATION; WATER; PROTOZOAN; BACTERIA; SPORES;
D O I
10.1111/j.1365-2672.2009.04168.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: In this study, we propose (i) to study the photodynamic inactivation (PDI) efficiency of neutral and cationic porphyrin derivatives, (ii) to characterize the kinetics of the inactivation process using Bacillus cereus as a model endospore-producing bacterium and (iii) to conclude on the applicability of porphyrin derivatives in the inactivation of bacterial endospores. Methods and Results: The study of PDI of Bacillus cereus endospores, taken as model-endospores, using porphyrin derivatives differing in the number of positive charges and in the meso-substituent groups, showed that neutral, mono-cationic and dicationic porphyrins are quite ineffective, in contrast with the tri- and tetra-cationic molecules. The most effective porphyrin is a tricationic porphyrin with a meso-pentafluorophenyl group. With this photosensitizer (PS), at 0.5 mu mol l(-1), a reduction of 3.5 log units occurs after only 4 min of irradiation. None of the porphyrin derivatives showed toxicity in the absence of light. Conclusions: Some porphyrin derivatives are efficient PSs for the inactivation of bacterial endospores and should be considered in further studies. Small modifications in the substituent groups, in addition to charge, significantly improve the effectiveness of the molecule as a PS for endospore inactivation. Significance and Impact of the Study: Tetrapyrrolic macrocycles should be regarded as worthy to explore for the PDI of spore-producing gram-positive bacteria. The development of molecules, more selective and effective, emerges as a new objective.
引用
收藏
页码:1986 / 1995
页数:10
相关论文
共 31 条
[1]  
Bonnett R., 2000, Chemical Aspects of Photodynamic Therapy
[2]  
Caminos D.A., 2005, Photochem Photobiol Sci, V5, P56
[3]   Photoinactivation of bacteria in wastewater by porphyrins:: Bacterial β-galactosidase activity and leucine-uptake as methods to monitor the process [J].
Carvalho, Carla M. B. ;
Gomes, Ana T. P. C. ;
Fernandes, Sonia C. D. ;
Prata, Ana C. B. ;
Almeida, Maria A. ;
Cunha, Maria A. ;
Tome, Joao P. C. ;
Faustino, Maria A. F. ;
Neves, Maria G. P. M. S. ;
Tome, Augusto C. ;
Cavaleiro, Jose A. S. ;
Lin, Zhi ;
Rainho, Jose P. ;
Rocha, Joao .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2007, 88 (2-3) :112-118
[4]   Sewage bacteriophage photoinactivation by cationic porphyrins:: a study of charge effect [J].
Costa, Liliana ;
Alves, Eliana ;
Carvalho, Carla M. B. ;
Tome, Joao P. C. ;
Faustino, Maria A. F. ;
Neves, Maria G. P. M. S. ;
Tome, Augusto C. ;
Cavaleiro, Jose A. S. ;
Cunha, Angela ;
Almeida, Adelaide .
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2008, 7 (04) :415-422
[5]   Photodynamic inactivation of Bacillus spores, mediated by phenothiazinium dyes [J].
Demidova, TN ;
Hamblin, MR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :6918-6925
[6]   Effect of cell-photo sensitizer binding and cell density on microbial photoinactivation [J].
Demidova, TN ;
Hamblin, MR .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (06) :2329-2335
[7]   Control of aerosol contaminants in indoor air: Combining the particle concentration reduction with microbial inactivation [J].
Grinshpun, Sergey A. ;
Adhikari, Atin ;
Honda, Takeshi ;
Kim, Ki Youn ;
Toivola, Mika ;
Rao, K. S. Ramchander ;
Reponen, Tiina .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (02) :606-612
[8]   Photodynamic therapy: a new antimicrobial approach to infectious disease? [J].
Hamblin, MR ;
Hasan, T .
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2004, 3 (05) :436-450
[9]   Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: A review [J].
Hijnen, WAM ;
Beerendonk, EF ;
Medema, GJ .
WATER RESEARCH, 2006, 40 (01) :3-22
[10]   Distribution of sewage indicated by Clostridium perfringens at a deep-water disposal site after cessation of sewage disposal [J].
Hill, RT ;
Straube, WL ;
Palmisano, AC ;
Gibson, SL ;
Colwell, RR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1741-1746