Water-soluble polysaccharides with pharmaceutical importance from Durian rinds (Durio zibethinus Murr.):: isolation, fractionation, characterisation and bioactivity

被引：90

作者：

Hokputsa, S

Gerddit, W

Pongsamart, S

Inngjerdingen, K

Heinze, T

Koschella, A

Harding, SE

Paulsen, BS

机构：

[1] Univ Nottingham, Sch Biosci, NCMH Phys Biochem Lab, Loughborough LE12 5RD, Leics, England

[2] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem, Bangkok 10330, Thailand

[3] Univ Oslo, Inst Pharm, Dept Pharmacognosy, N-0316 Oslo, Norway

[4] Univ Jena, Kompetenzzentrum Polysaccharidforsch, D-07743 Jena, Germany

来源：

CARBOHYDRATE POLYMERS | 2004年 / 56卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Durio zibethinus; durian; pectic polysaccharide;

D O I：

10.1016/j.carbpol.2004.03.018

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Crude, pharmaceutically useful water-soluble polysaccharides have been isolated from durian rinds (Durio zibethinus) by hot water extraction followed by ethanol precipitation. The polysaccharides were fractionated by anion exchange chromatography and size exclusion chromatography. Characterisation of the sub-fractions by methanolysis, methylation analysis and NMR spectroscopy revealed that the principal components were pectic polysaccharides with starch as a contaminant. Physical features namely molecular weight (M-w) and intrinsic viscosity of the main fractions were investigated by size exclusion chromatography coupled to multi angle laser light scattering (SEC/MALLS) and capillary viscometer, respectively. The main fractions were subjected to the complement-fixation assay and the relationship of the chemical features of the polysaccharide, fractions with their activity was also considered. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：471 / 481

页数：11