Characterization of di-, tri- and tetranucleotide microsatellite markers with perfect repeats for Trypanosoma brucei and related species

被引：15

作者：

Balmer, Oliver

Palma, Christopher

Macleod, Annette

Caccone, Adalgisa

机构：

[1] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT 06520 USA

[2] Univ Glasgow, Wellcome Ctr Mol Parasitol, Glasgow, Lanark, Scotland

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期

基金：

英国惠康基金;

关键词：

coinfection; cross-amplification; kinetoplastid; microsatellites; population genetics; Trypanosoma;

D O I：

10.1111/j.1471-8286.2006.01297.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trypanosoma brucei, a unicellular parasite causing human sleeping sickness and animal nagana, has a great impact on the socioeconomic environment of sub-Saharan Africa. The dynamics of the parasite are still poorly understood. We have characterized 14 polymorphic di-, tri- and tetranucleotide microsatellite loci with perfect repeats (only one motif) exhibiting between five and 16 alleles in T. brucei isolates from all over Africa and from all described subspecies. The microsatellites will be useful in addressing population genetic questions in T. brucei to better understand the population structure and spread of this important parasite.

引用

页码：508 / 510

页数：3

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