Massively parallel signature sequencing profiling of fetal human neural precursor cells

被引：13

作者：

Cai, JL

Shin, S ^{[1
]}

Wright, L

Liu, Y

Zhou, DX

Xue, HP

Khrebtukova, I

Mattson, MP

Svendsen, CN

Rao, MS

机构：

[1] NIA, Stem Cell Biol Unit, Neurosci Lab, Intramural Res Program,NIH, Baltimore, MD 21224 USA

[2] Univ Wisconsin, Waisman Ctr, Madison, WI 53705 USA

[3] Invitrogen Corp, Corp Res Labs, Carlsbad, CA 92008 USA

[4] Johns Hopkins Sch Med, Baltimore, MD 21287 USA

[5] Solexa Inc, Hayward, CA 94545 USA

来源：

STEM CELLS AND DEVELOPMENT | 2006年 / 15卷 / 02期

关键词：

D O I：

10.1089/scd.2006.15.232

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

We have examined gene expression in multipotent neural precursor cells (NPCs) derived from human fetal (f) brain tissue and compared its expression profiles with embryonic stem (ESC) cells, embryoid body cell (EBC), and astrocyte precursors using the technique of massively parallel signature sequencing (MPSS). Gene expression profiles show that fNPCs express core neural stem cells markers and share expression profiles with astrocyte precursor cells (APCs) rather than ESC or EBC. Gene expression analysis shows that fNPCs differ from other adult stem and progenitor cells in their marker expression and activation of specific functional networks such as the transforming growth factor beta (TGF beta) and Notch signaling pathways. In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs.

引用

收藏

页码：232 / 244

页数：13

相关论文

共 18 条

[1]

Stage-specific changes in gene expression in acutely isolated mouse CNS progenitor cells [J].

Abramova, N ;

Charniga, C ;

Goderie, SK ;

Temple, S .

DEVELOPMENTAL BIOLOGY, 2005, 283 (02) :269-281

[2]

The significance of digital gene expression profiles [J].

Audic, S ;

Claverie, JM .

GENOME RESEARCH, 1997, 7 (10) :986-995

[3]

In vitro cloning of complex mixtures of DNA on microbeads:: Physical separation of differentially expressed cDNAs [J].

Brenner, S ;

Williams, SR ;

Vermaas, EH ;

Storck, T ;

Moon, K ;

McCollum, C ;

Mao, JI ;

Luo, SJ ;

Kirchner, JJ ;

Eletr, S ;

DuBridge, RB ;

Burcham, T ;

Albrecht, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) :1665-1670

[4]

Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays [J].

Brenner, S ;

Johnson, M ;

Bridgham, J ;

Golda, G ;

Lloyd, DH ;

Johnson, D ;

Luo, SJ ;

McCurdy, S ;

Foy, M ;

Ewan, M ;

Roth, R ;

George, D ;

Eletr, S ;

Albrecht, G ;

Vermaas, E ;

Williams, SR ;

Moon, K ;

Burcham, T ;

Pallas, M ;

DuBridge, RB ;

Kirchner, J ;

Fearon, K ;

Mao, J ;

Corcoran, K .

NATURE BIOTECHNOLOGY, 2000, 18 (06) :630-634

[5]

Prox1 function controls progenitor cell proliferation and horizontal, cell genesis in the mammalian retina [J].

Dyer, MA ;

Livesey, FJ ;

Cepko, CL ;

Oliver, G .

NATURE GENETICS, 2003, 34 (01) :53-58

[6]

The close homologue of the neural adhesion molecule L1 (CHL1): patterns of expression and promotion of neurite outgrowth by heterophilic interactions [J].

Hillenbrand, R ;

Molthagen, M ;

Montag, D ;

Schachner, M .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (03) :813-826

[7]

Kalyani AJ, 1998, J NEUROSCI, V18, P7856

[8]

Molecular markers in CNS stem cell research: hitting a moving target [J].

Kornblum, HI ;

Geschwind, DH .

NATURE REVIEWS NEUROSCIENCE, 2001, 2 (11) :843-846

[9]

CD44 expression identifies astrocyte-restricted precursor cells [J].

Liu, Y ;

Han, SSW ;

Wu, YY ;

Tuohy, TMF ;

Xue, HP ;

Cai, JL ;

Back, SA ;

Sherman, LS ;

Fischer, I ;

Rao, MS .

DEVELOPMENTAL BIOLOGY, 2004, 276 (01) :31-46

[10]

Monitoring early differentiation events in human embryonic stem cells by massively parallel signature sequencing and expressed sequence tag scan [J].

Miura, T ;

Luo, YQ ;

Khrebtukova, I ;

Brandenberger, R ;

Zhou, DX ;

Thies, RS ;

Vasicek, T ;

Young, H ;

Lebkowski, J ;

Carpenter, MK ;

Rao, MS .

STEM CELLS AND DEVELOPMENT, 2004, 13 (06) :694-715