On-probe digestion of bacterial proteins for MALDI-MS

被引:37
作者
Harris, WA [1 ]
Reilly, JP [1 ]
机构
[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA
关键词
D O I
10.1021/ac025636i
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
On-probe digestion combined with MALDI mass spectrometry is studied as a rapid method for the analysis and identification of bacterial proteins. The use of trypsin adsorbed to the probe surface reduces the digestion time from hours to minutes. A high amount of trypsin must be applied to the probe for the successful digestion of bacterial proteins. Mass spectra of the digest contain a number of low-mass digest fragments. Several components of a B. subtilis bacterial digest can be identified through postsource decay and database searching.
引用
收藏
页码:4410 / 4416
页数:7
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