Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein

被引：20

作者：

Bridges, Dave

MacDonald, Justin A.

Wadzinski, Brian

Moorhead, Greg B. G.

机构：

[1] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 4N1, Canada

[2] Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada

[3] Vanderbilt Univ, Med Ctr, Dept Pharmacol, Nashville, TN 37232 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 346卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

protein phosphorylation; protein kinase A; AKAP; protein phosphatase 1; adipocytes; lipolysis; lipid droplets; D-AKAP1;

D O I：

10.1016/j.bbrc.2006.05.138

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase A (PKA) plays an important role in the regulation of lipid metabolism in adipocytes. The activity of PKA is known to be modulated by its specific location in the cell, a process mediated by A-kinase anchoring proteins (AKAPs). In order to examine the subcellular localization of PKA in this tissue we performed a search for AKAP proteins in adipocytes. We purified a 120 kDa protein which can bind both the regulatory subunit of PKA as well as the catalytic subunit of protein phosphatase 1 (PP1). This protein was found to be enriched in the lipid droplet fraction of primary adipocytes and was identified as D-AKAP1. This protein may play an important role in the regulation of PKA in adipocytes. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：351 / 357

页数：7

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