Application of Ultra Performance Liquid Chromatography-Mass Spectrometry to Profiling Rat and Dog Bile

被引：53

作者：

Plumb, Robert S. ^{[2
]}

Rainville, Paul D. ^{[2
]}

Potts, Warren B., III ^{[2
]}

Johnson, Kelly A. ^{[2
]}

Gika, Eleni ^{[1
]}

Wilson, Ian D. ^{[1
]}

机构：

[1] AstraZeneca, Dept Clin Pharmacol Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[2] Waters Corp, Pharmaceut Business Operat, Milford, MA USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 05期

关键词：

bile; metabolism; liquid chromatography; mass spectrometry; biofluid profiling; metabolomics; metabonomics; PEAK-CAPACITY; METABONOMICS; URINE; ACIDS; METABOLOMICS; INFORMATION; TEMPERATURE; SYSTEMS; WATER; TOOL;

D O I：

10.1021/pr801078a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed-phase gradient UPLC-ESI-MS, in both positive and negative ionization modes, has been applied to the analysis of untreated bile obtained from bile-cannulated rats and dogs. The use of UPLC provided a high-resolution system that enabled global metabolite profiles of bile from the two species to be obtained that were suitable for metabolomic and metabonomic applications. When these metabolite profiles were analyzed using unsupervised multivariate statistical methods, based on principle components analysis (PCA), they were correctly classified by species of origin. Conventional approaches to characterizing sample components via, for example, mass and retention time compared to authentic standards resulted in the identification of a range of bile acids. In addition, the value of using an "MSE" approach to simplify the problem of classifying and identifying the metabolites present in the sample (as e.g., sulfates or taurine conjugates) was demonstrated.

引用

页码：2495 / 2500

页数：6

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