Temperature as a variable in liquid chromatography: Development and application of a model for the separation of model drugs using water as the eluent

被引：41

作者：

Edge, A. M. ^{[1
]}

Shillingford, S. ^{[1
]}

Smith, C. ^{[1
]}

Payne, R. ^{[1
]}

Wilson, I. D. ^{[1
]}

机构：

[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1132卷 / 1-2期

关键词：

high temperature chromatography; HPLC; ultra performance liquid chromatography; UPLC;

D O I：

10.1016/j.chroma.2006.07.081

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of high temperatures in liquid chromatography allows for the use of a purely aqueous mobile phase. At elevated temperatures water possesses many of the characteristics of organic solvents in terms of eluotropic strength, as well as having a lower viscosity. A model is developed, based on data obtained using a range of model drugs, which demonstrates the relationship between temperature, flow and pressure. Experimental data from different column types, at temperatures from 40 degrees C to 180 degrees C, is presented which matches well with the predicted data from the model. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：206 / 210

页数：5

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