An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+T cell responses in a mouse model

被引：130

作者：

Chahal, Jasdave S. ^{[1
,2
]}

Fang, Tao ^{[1
]}

Woodham, Andrew W. ^{[1
]}

Khan, Omar F. ^{[2
]}

Ling, Jingjing ^{[1
]}

Anderson, Daniel G. ^{[2
]}

Ploegh, Hidde L. ^{[1
,3
]}

机构：

[1] Cambridge Ctr 9, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02142 USA

[3] Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

美国国家卫生研究院;

关键词：

CD8(+) T-CELLS; DENGUE VIRUS; PEPTIDE; GLYCOPROTEIN; REACTIVITY; PROTECTION; INFECTION; BINDING; KB;

D O I：

10.1038/s41598-017-00193-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV's neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/ 6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.

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