Pre-Existing Hypertension Dominates γδT Cell Reduction in Human Ischemic Stroke

被引:17
作者
Adamski, Mateusz G. [1 ,2 ]
Li, Yan [1 ]
Wagner, Erin [1 ]
Yu, Hua [1 ]
Seales-Bailey, Chloe [1 ]
Durkin, Helen [3 ]
Hao, Qing [1 ]
Soper, Steven A. [4 ]
Murphy, Michael [5 ]
Baird, Alison E. [1 ]
机构
[1] Suny Downstate Med Ctr, Dept Neurol, Brooklyn, NY 11203 USA
[2] Jagiellonian Univ, Coll Med, Dept Neurol, Krakow, Poland
[3] Suny Downstate Med Ctr, Dept Pathol, Brooklyn, NY 11203 USA
[4] Univ N Carolina, Chapel Hill, NC USA
[5] Louisiana State Univ, Baton Rouge, LA 70803 USA
来源
PLOS ONE | 2014年 / 9卷 / 05期
关键词
INTERFERON-GAMMA; ESCHERICHIA-COLI; GENE-EXPRESSION; BLOOD-PRESSURE; INTERLEUKIN-17; LYMPHOCYTES; AGE; MECHANISMS; GENERATION; INFECTION;
D O I
10.1371/journal.pone.0097755
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
T lymphocytes may play an important role in the evolution of ischemic stroke. Depletion of gamma delta T cells has been found to abrogate ischemia reperfusion injury in murine stroke. However, the role of gamma delta T cells in human ischemic stroke is unknown. We aimed to determine gamma delta T cell counts and gamma delta T cell interleukin 17A (IL-17A) production in the clinical setting of ischemic stroke. We also aimed to determine the associations of gamma delta T cell counts with ischemic lesion volume, measures of clinical severity and with major stroke risk factors. Peripheral blood samples from 43 acute ischemic stroke patients and 26 control subjects matched on race and gender were used for flow cytometry and complete blood count analyses. Subsequently, cytokine levels and gene expression were measured in gamma delta T cells. The number of circulating gamma delta T cells was decreased by almost 50% (p = 0.005) in the stroke patients. gamma delta T cell counts did not correlate with lesion volume on magnetic resonance diffusion-weighted imaging or with clinical severity in the stroke patients, but gamma delta T cells showed elevated levels of IL-17A (p = 0.048). Decreased gamma delta T cell counts were also associated with older age (p = 0.004), pre-existing hypertension (p = 0.0005) and prevalent coronary artery disease (p = 0.03), with pre-existing hypertension being the most significant predictor of gamma delta T cell counts in a multivariable analysis. gamma delta T cells in human ischemic stroke are reduced in number and show elevated levels of IL-17A. A major reduction in gamma delta T lymphocytes also occurs in hypertension and may contribute to the development of hypertension-mediated stroke and vascular disease.
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页数:9
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