Transposon-like structure of a new plasmid-encoded restriction-modification system in Rhizobium leguminosarum VF39SM

被引:28
作者
Rochepeau, P
Selinger, LB
Hynes, MF
机构
[1] UNIV CALGARY,DEPT BIOL SCI,CALGARY,AB T2N 1N4,CANADA
[2] AGR CANADA,RES STN,LETHBRIDGE,AB T1J 4B1,CANADA
来源
MOLECULAR & GENERAL GENETICS | 1997年 / 256卷 / 04期
基金
加拿大自然科学与工程研究理事会;
关键词
Rhizobium; restriction-modification system; insertion sequence; transposon;
D O I
10.1007/s004380050582
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Total DNA isolated from Rhizobium leguminosarum VF39SM cells is resistant to cleavage by the restriction endonuclease PstI. Plasmid curing and transfer studies localized this phenotype to pRleVF39b, the second smallest of six plasmids found in this bacterium. In vitro selection for vector modification was employed to isolate a presumptive methylase gene (M.Rle39BI) from a plasmid gene library. Total and plasmid DNAs isolated from E. coli containing M.RleBI were resistant to digestion by PstI. Sequence data suggested that a putative restriction endonuclease (R.Rle39BI) was also encoded on the same fragment. The two genes were flanked by identical copies of a putative insertion sequence, which was also present in several copies elsewhere in the VF39SM genome. The presence of this element in other strains examined suggested that this element is indeed an insertion sequence. The differences in G/C content between the DNA coding for the R/M system and that of the IS element suggest that this DNA region may have been acquired by horizontal transfer.
引用
收藏
页码:387 / 396
页数:10
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