Capacitive sensor for lipolytic enzymes

被引：29

作者：

Mirsky, VM

Krause, C

Heckmann, KD

机构：

[1] Analytical Chemistry, Chemical Sensors and Biosensors, University of Regensburg, Regensburg 93053

来源：

THIN SOLID FILMS | 1996年 / 284卷

关键词：

phospholipase A(2); enzyme assay; capacitive sensor; self-assembly layer;

D O I：

10.1016/S0040-6090(95)08485-1

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The preparation of the capacitive biosensor for monitoring the activity of enzymes (lipases, phospholipases, etc.) hydrolysing water-insoluble substrates into water-soluble products is described. The sensor is based on a sandwich-like structure: Au/S(CH2)(17)CH3/substrate/electrolyte. Hydrolysis of the substrate leads to the formation of water-soluble products and desorption of these compounds from the electrode. When the product formation is the rate-limiting step of this process, the desorption rate is determined by the enzyme activity. The desorption can be easily monitored as an increase of the electrode capacitance. By following this principle it was possible to develop a sensor monitoring phospholipase A, activity even at the 50 pg ml(-1) level.

引用

页码：939 / 941

页数：3

共 7 条

[1]

KRAUSE C, UNPUB LANGMUIR

[2] ELECTROSTATIC ASSAY OF PHOSPHOLIPASE-A ACTIVITY - AN APPLICATION OF THE 2ND HARMONIC METHOD OF MONITORING MEMBRANE BOUNDARY POTENTIALS [J].