Murine spermatogonial stem cells: targeted transgene expression and purification in an active state

被引：91

作者：

Giuili, G

Tomljenovic, A

Labrecque, N

Oulad-Abdelghani, M

Rassoulzadegan, M

Cuzin, F ^{[1
]}

机构：

[1] Univ Nice, INSERM, U470, F-06108 Nice 2, France

[2] Inst Genet & Biol Mol & Cellulaire, Strasbourg, France

来源：

EMBO REPORTS | 2002年 / 3卷 / 08期

关键词：

D O I：

10.1093/embo-reports/kvf149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 400 bp fragment of the spermatogonia-specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP-Cam, and the beta(1)- and alpha(6)-integrins characteristic of the stem cell fraction. A 700-fold enrichment in stem cells was determined by the ability of the purified fractions to re-establish spermatogenesis in germ cell-depleted recipient testes.

引用

页码：753 / 759

页数：7

共 20 条

[1]

Anderson R, 1999, J REPROD FERTIL, V116, P379, DOI 10.1530/jrf.0.1160379

[2]

[Anonymous], 1994, MANIPULATING MOUSE E

[3] INTERNALIZATION OF THE HUMAN IMMUNODEFICIENCY VIRUS DOES NOT REQUIRE THE CYTOPLASMIC DOMAIN OF CD4 [J].