TRANSACTIVATION OF THE PROMOTERLESS LUCIFERASE-ENCODING VECTORS PXP1 AND PXP2 BY C/EBP-ALPHA

被引：17

作者：

LI, YC

HAYES, S

YOUNG, AP

机构：

[1] OHIO STATE UNIV,OHIO STATE BIOTECHNOL CTR,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,MOLEC CELLULAR & DEV BIOL PROGRAM,COLUMBUS,OH 43210

[3] OHIO STATE UNIV,COLL PHARM,DIV PHARMACOL,COLUMBUS,OH 43210

来源：

GENE | 1994年 / 138卷 / 1-2期

关键词：

RECOMBINANT DNA; TRANSIENT GENE EXPRESSION; CRYPTIC PROMOTERS; REPORTER GENE; ELECTROPORATION;

D O I：

10.1016/0378-1119(94)90819-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Transactivation of the firefly luciferase-encoding vectors, pXP1 and pXP2, by the or isoform of CCAAT/enhancer-binding protein (C/EBP) is reported. Thus, these vectors are not 'promoterless' in every cellular context, and transactivation by C/EBP or a closely related factor should be considered as a possible explanation for the relatively high background levels of luciferase production that are occasionally observed after transfection of certain cells with these or other similar vectors.

引用

页码：257 / 258

页数：2

共 8 条

[1] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].