Effects of oxidative stress on chlorophyll biosynthesis in cucumber (Cucumis sativus) cotyledons

被引:89
作者
Aarti, P. D. [1 ]
Tanaka, Ryouichi [1 ]
Tanaka, Ayumi [1 ]
机构
[1] Hokkaido Univ, Inst Low Temp Sci, Sapporo, Hokkaido 0600819, Japan
关键词
IX MONOMETHYL ESTER; PHOTOOXIDATIVE STRESS; 5-AMINOLEVULINIC ACID; PROTOPORPHYRIN-IX; CHLOROPLASTS; ACCUMULATION; INDUCTION; PARAQUAT; SYSTEM; LEAVES;
D O I
10.1111/j.1399-3054.2006.00720.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We conducted a series of experiments to assess the effects of oxidative stress on chlorophyll biosynthesis in the vascular plant Cucumis sativus (cucumber). Specifically, cucumber cotyledons were treated with 100 mu M methyl viologen (MV) and subsequently exposed to dark (0 mu E m(-2) s(-1)), low light (4045 mu E m(-2) s(-1)), or high light (1500-1600 mu E m(-2) s(-1)). Following treatment, extracts of these samples were subjected to high-performance liquid chromatography (HPLC) to quantitate the accumulation of chlorophyll biosynthetic pathway intermediates. The results of these analyses revealed significant accumulation of Mg-protoporphyrin IX monomethyl ester (Mg-proto IX ME) in green (14-h illuminated) as well as in etiolated cotyledons with MV treatment. These data suggest that MV-induced oxidative stress may have inhibited Mg-proto IX ME cyclase activity. Upon exposure to high light, in the presence or absence of MV, both green and etiolated cotyledons predominantly accumulated protoporphyrin IX (Proto IX). These elevated levels of Proto IX might be attributable to attenuated activity of any or all of the following enzymes: Mg-chelatase, Fe-chelatase and protoporphyrinogen IX oxidase. We also observed that MV-induced oxidative stress impacts on chlorophyll biosynthesis to a greater extent than on photosystem II. These results demonstrate that oxidative stress impedes key steps in chlorophyll biosynthesis by either directly or indirectly inhibiting the activity of these enzymes.
引用
收藏
页码:186 / 197
页数:12
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