Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy

被引:101
作者
Wurpel, GWH [1 ]
Schins, JM [1 ]
Müller, M [1 ]
机构
[1] Univ Amsterdam, Swammerdam Inst Life Sci, NL-1090 GB Amsterdam, Netherlands
关键词
D O I
10.1364/OL.27.001093
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate the three-dimensional (3D) imaging capabilities and chemical specificity of multiplex coherent anti-Stokes Raman scattering microscopy. The simultaneous acquisition of a significant part of the vibrational spectrum at each specimen position permits straightforward differentiation among chemical species. 3D imaging is illustrated with a lipid multilamellar vesicle, and lateral and axial resolutions are determined. (C) 2002 Optical Society of America.
引用
收藏
页码:1093 / 1095
页数:3
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